EMBRYOLOGICS
Registry

Registry · media · CooperSurgical (Origio)

ICSI Cumulase (recombinant hyaluronidase)

Recombinant human hyaluronidase for removing cumulus cells before ICSI.

Clinical review pending·1 min read·Updated 2026-08-16

What it is and how it's used

Enzymatic step before mechanical denudation. Exposure time and concentration matter: too long damages the oolemma and raises degeneration after ICSI; too short leaves cumulus and makes injection harder.

Typical failure modes

  • Higher post-ICSI degeneration after a lot change or longer exposure.
  • Poor fertilisation with fragile-looking oocytes.

What to check first

  1. Exposure time in seconds actually used vs IFU/SOP.
  2. Working concentration and dilution practice.
  3. Lot and storage.

IFU and practice notes

Follow the IFU for concentration and time; train denudation with a stopwatch.

Neutral, and the IFU wins

This page summarises common experience across labs and is not manufacturer guidance. Always follow the current instructions for use for the exact product and version you have, and validate any change of product or protocol before patient use. Corrections and additions are welcome — tell us.

This page is educational and general. It is not a substitute for advice from your own clinic or the manufacturer's instructions for use.