Registry · media · CooperSurgical (SAGE)
SAGE 1-Step (single-step culture)
Single-step medium for continuous culture from fertilisation to blastocyst.
What it is and how it's used
Uninterrupted culture, so incubator stability, drop volume and osmolality drive outcomes.
Typical failure modes
- Osmolality creep in dry incubators over 5–6 days.
- Lot-related quality change.
What to check first
- Post-culture osmolality spot check.
- Humidity and drop volume.
- Lot vs KPI change.
IFU and practice notes
Follow the IFU; confirm humidity requirements for your incubator.
Neutral, and the IFU wins
This page summarises common experience across labs and is not manufacturer guidance. Always follow the current instructions for use for the exact product and version you have, and validate any change of product or protocol before patient use. Corrections and additions are welcome — tell us.
Shared with you by your clinic · Source: https://embryologics.com/embryologists/registry/sage-1-step · Medical Interventions. Reviewed by a certified clinical embryologist. Educational information — please discuss your own situation with your clinic.
This page is educational and general. It is not a substitute for advice from your own clinic or the manufacturer's instructions for use.