EMBRYOLOGICS
Registry

Registry · consumable · Kitazato

Cryotop (vitrification device)

Open vitrification device: a thin polypropylene strip on which the embryo is loaded in a minimal volume, then plunged into LN₂.

Clinical review pending·1 min read·Updated 2026-08-16

What it is and how it's used

Loading volume and speed determine cooling rate. Open system means direct LN₂ contact (very fast cooling) and requires clean LN₂ practice.

Typical failure modes

  • Ice formation from too much medium around the embryo.
  • Device lot variation.
  • Slow plunge or vapour pause reducing cooling rate.
  • Loss of embryo during loading/unloading with poor pipette control.

What to check first

  1. Loading volume technique (video review).
  2. Plunge directness; LN₂ level in the vessel.
  3. Device lot vs survival cohort.

IFU and practice notes

Follow the IFU; retrain loading with practice material regularly.

Neutral, and the IFU wins

This page summarises common experience across labs and is not manufacturer guidance. Always follow the current instructions for use for the exact product and version you have, and validate any change of product or protocol before patient use. Corrections and additions are welcome — tell us.

This page is educational and general. It is not a substitute for advice from your own clinic or the manufacturer's instructions for use.