Registry · media · Vitrolife
G-TL (single-step, time-lapse culture)
Single-step medium designed for uninterrupted culture from fertilisation to blastocyst, typically in time-lapse incubators.
What it is and how it's used
One medium, no changeover: dishes are prepared once and embryos left undisturbed for 5–6 days. Reduces handling variables and makes incubator stability and drop osmolality the dominant factors.
Typical failure modes
- Osmolality rise over 5–6 days in dry incubators or small drops → slowed development, poorer blastocysts.
- Lot-related changes in blastocyst quality without changes in fertilisation.
- Prolonged pre-equilibration leading to evaporation before embryos are even loaded.
What to check first
- Osmolality of medium after the full culture period in the actual incubator (spot check).
- Humidification status of the incubator; drop volume; oil volume and quality.
- Lot number versus the KPI change; storage conditions.
IFU and practice notes
Follow the IFU for drop volume, overlay, equilibration and culture duration; verify humidity requirements for your incubator model.
Neutral, and the IFU wins
This page summarises common experience across labs and is not manufacturer guidance. Always follow the current instructions for use for the exact product and version you have, and validate any change of product or protocol before patient use. Corrections and additions are welcome — tell us.
Shared with you by your clinic · Source: https://embryologics.com/embryologists/registry/vitrolife-g-tl · Medical Interventions. Reviewed by a certified clinical embryologist. Educational information — please discuss your own situation with your clinic.
This page is educational and general. It is not a substitute for advice from your own clinic or the manufacturer's instructions for use.