Registry · technique · —
ICSI (intracytoplasmic sperm injection) — technique
Injection of a single immobilised sperm into a mature oocyte using micromanipulators.
What it is and how it's used
Operator-dependent. Consistency of oocyte orientation, membrane breakage confirmation, injection depth and speed determine fertilisation and degeneration.
Typical failure modes
- Fertilisation drop or degeneration rise with a new/returning operator, new pipette lot, or changed PVP/immobilisation practice.
- Failure to break the oolemma (no activation) or excessive aspiration (damage).
What to check first
- Split fertilisation and degeneration by operator.
- Pipette lot; PVP/hyaluronan drop age; heated stage temperature by probe.
- Video review of technique against SOP.
IFU and practice notes
Maintain competency records and periodic peer review; use manufacturer IFU for pipettes and media.
Neutral, and the IFU wins
This page summarises common experience across labs and is not manufacturer guidance. Always follow the current instructions for use for the exact product and version you have, and validate any change of product or protocol before patient use. Corrections and additions are welcome — tell us.
Shared with you by your clinic · Source: https://embryologics.com/embryologists/registry/icsi-technique · Medical Interventions. Reviewed by a certified clinical embryologist. Educational information — please discuss your own situation with your clinic.
This page is educational and general. It is not a substitute for advice from your own clinic or the manufacturer's instructions for use.